As part of the continuation of the ANR JCJC PlasmonISC project, we propose a thesis subject mainly experimental in nano-photonics. The objective of the thesis is to study the influence of a nano-antenna (plasmonic, magnetic or dielectric) on the rate governing the photophysics of fluorescence emission from a single molecule, with a particular interest in the intersystem crossing rate. We have developed a dedicated optical bench combining optical and atomic force microscopy, an experimental procedure, as well as signal processing tools, showing encouraging first results with a dielectric tip. We wish to continue to explore the single molecule/nano-antenna interaction with other types of tips generating other physical effects. The ability to control the transition to the triplet state is of great interest for single photon sources, organic light emitting diodes, and in chemistry.