General information
Organisation
The French Alternative Energies and Atomic Energy Commission (CEA) is a key player in research, development and innovation in four main areas :
• defence and security,
• nuclear energy (fission and fusion),
• technological research for industry,
• fundamental research in the physical sciences and life sciences.
Drawing on its widely acknowledged expertise, and thanks to its 16000 technicians, engineers, researchers and staff, the CEA actively participates in collaborative projects with a large number of academic and industrial partners.
The CEA is established in ten centers spread throughout France
Reference
SL-DRF-24-0519
Direction
DRF
Thesis topic details
Category
Life Sciences
Thesis topics
Localization and dynamics of key nucleoid-associated proteins during stress-induced bacterial nucleoid remodeling.
Contract
Thèse
Job description
Nucleoid remodeling, and in particular, nucleoid compaction, is a common stress response mechanism in bacteria that allows bacteria to rapidly respond to sudden changes in their environment. Using advanced optical microscopy approaches, we recently followed the changes in nucleoid shape and volume induced by exposure to intense UV-C light in the radiation resistant bacterium, Deinococcus radiodurans. This two-step process involves a rapid initial nucleoid condensation step followed by a slower decompaction phase to restore normal nucleoid morphology, before cell growth and division can resume. Nucleoid associated proteins (NAP) are known to be key players in this process, although the details of their implication remain largely elusive. We have started to shed light on the central role of the major NAP, the histone-like HU protein, in this process. The proposed PhD project will extend this work to the study of 5 additional NAPs involved in stress-induced nucleoid remodeling. The PhD student will perform biochemical studies to follow the abundance of these key factors, live cell imaging to map their distribution and single-particle tracking to determine their dynamics. This work will contribute to a better understanding of the fundamental processes that govern bacterial genome organisation and how they are affected by UV radiation and DNA damage.
University / doctoral school
Chimie et Sciences du Vivant (EDCSV)
Université Grenoble Alpes
Thesis topic location
Site
Grenoble
Requester
Position start date
01/10/2024
Person to be contacted by the applicant
TIMMINS JOANNA joanna.timmins@ibs.fr
CNRS
Institut de Biologie Structurale (IBS), UMR 5075 UGA,CNRS,CEA
71 AVENUE DES MARTYRS
CS 10090
38044 GRENOBLE CEDEX 9
04 57 42 86 78
Tutor / Responsible thesis director
TIMMINS JOANNA joanna.timmins@ibs.fr
CNRS
Institut de Biologie Structurale (IBS), UMR 5075 UGA,CNRS,CEA
71 AVENUE DES MARTYRS
CS 10090
38044 GRENOBLE CEDEX 9
04 57 42 86 78
En savoir plus
https://www.ibs.fr/fr/recherche/assemblage-dynamique-et-reactivite/groupe-imagerie-integree-de-la-reponse-au-stress/?lang=fr
https://www.ibs.fr/fr/recherche/assemblage-dynamique-et-reactivite/groupe-imagerie-integree-de-la-reponse-au-stress/equipe-genom/