General information
Organisation
The French Alternative Energies and Atomic Energy Commission (CEA) is a key player in research, development and innovation in four main areas :
• defence and security,
• nuclear energy (fission and fusion),
• technological research for industry,
• fundamental research in the physical sciences and life sciences.
Drawing on its widely acknowledged expertise, and thanks to its 16000 technicians, engineers, researchers and staff, the CEA actively participates in collaborative projects with a large number of academic and industrial partners.
The CEA is established in ten centers spread throughout France
Reference
SL-DRF-24-0875
Direction
DRF
Thesis topic details
Category
Life Sciences
Thesis topics
LIGHT-NMR: A POWERFUL TOOL FOR UNDERSTANDING AND IMPROVING THE PROPERTIES OF PHOTOSWITCHABLE FLUORESCENT PROTEINS
Contract
Thèse
Job description
The recruited student will investigate the photophysical mechanisms of reversibly photoswitchable fluorescent proteins (RSFPs) employing solution NMR spectroscopy coupled with in-situ illumination and variable oxygen pressure. RSFPs are capable to switch between a fluorescent on-state and a nonfluorescent off-state under specific light illumination, and have fostered many types of imaging applications including super-resolution methods. Multidimensional NMR spectroscopy is a particularly powerful atomic resolution technique providing detailed information on conformational protein dynamics, as well as the local chemistry (protonation states, H-bonding interactions, …) involved in the photophysics of the chomophore within the protein scaffold. In the proposed PhD project, we intend to further improve our NMR in-situ illumination device by adding capabilities such as additional wavelengths of emitting light sources, fluorescence detection, and oxygen pressure control. This will allow to directly correlate the conformational dynamics of various states with their photophysical properties, as well as the effect of oxygen on triplet state formation and photobleaching. We will apply this NMR methodology to several green and red model RSFPs, as well as FAST systems. The goal will be to contribute fundamental knowledge of these fluorescent markers and to design improved variants.
University / doctoral school
Ecole Doctorale de Physique de Grenoble (EdPHYS)
Université Grenoble Alpes
Thesis topic location
Site
Grenoble
Requester
Position start date
01/09/2024
Person to be contacted by the applicant
BRUTSCHER Bernhard bernhard.brutscher@ibs.fr
CEA
DRF/IRIG//IBS
IBS
UMR 5075
71 rue Jules Horowitz
38044 Grenoble CEDEX 9
0457428562
Tutor / Responsible thesis director
BRUTSCHER Bernhard bernhard.brutscher@ibs.fr
CEA
DRF/IRIG//IBS
IBS
UMR 5075
71 rue Jules Horowitz
38044 Grenoble CEDEX 9
0457428562
En savoir plus
https://www.ibs.fr/fr/recherche/assemblage-dynamique-et-reactivite/groupe-de-rmn-biomoleculaire/