General information
Organisation
The French Alternative Energies and Atomic Energy Commission (CEA) is a key player in research, development and innovation in four main areas :
• defence and security,
• nuclear energy (fission and fusion),
• technological research for industry,
• fundamental research in the physical sciences and life sciences.
Drawing on its widely acknowledged expertise, and thanks to its 16000 technicians, engineers, researchers and staff, the CEA actively participates in collaborative projects with a large number of academic and industrial partners.
The CEA is established in ten centers spread throughout France
Reference
SL-DRF-24-0310
Direction
DRF
Thesis topic details
Category
Life Sciences
Thesis topics
[FeFe]-hydrogenase active site assembly machinery
Contract
Thèse
Job description
To tackle the climate crisis, humanity urgently needs renewable and decarbonized energy sources. A promising solution lies in harnessing dihydrogen (H2), and enzymes known as [FeFe] hydrogenases can play a vital role in its production. These enzymes catalyze the reversible oxidation of dihydrogen, employing an active site called the 'H-cluster,' a complex organometallic structure. The intricate biosynthesis of this cluster involves three maturation proteins: HydG, HydE, and HydF. Despite recent progress, a full understanding of this process remains elusive due to the complexity of the chemical reactions involved. Our goal is to conduct a structural study combined with step-by-step reaction monitoring using spectroscopy. This approach aims to identify and characterize various reaction intermediates of one key enzyme in the process. This collaborative project involves two leading CEA teams specializing in the study of oxygen-sensitive metalloproteins. The doctoral student will benefit from an ideal scientific and technical environment to achieve this objective, crucially important for advancing hydrogen economy development.
University / doctoral school
Chimie et Sciences du Vivant (EDCSV)
Université Grenoble Alpes
Thesis topic location
Site
Grenoble
Requester
Position start date
01/10/2024
Person to be contacted by the applicant
NICOLET Yvain yvain.nicolet@ibs.fr
CEA
DRF/IRIG//IBS
Institut de Biologie Structurale
EPN Campus, CS 10090
71 avenue des Martyrs
F-38044 Grenoble Cedex 9 - France
+33457428603
Tutor / Responsible thesis director
GAMBARELLI Serge serge.gambarelli@cea.fr
CEA
DRF/IRIG//SYMMES
CEA/Grenoble
04 38 78 39 40
En savoir plus
https://www.ibs.fr/en/research/assembly-dynamics-and-reactivity/metalloproteins-group-y-nicolet/